GingaNinga

mine has said that all the ink is critically low and I’ve just ignored it for the past few months and it just keeps going.

In animal research we often refer to genotype and phenotype. Genotype refers to the set of genes the animals the animals carry (what they are capable of expressing) and phenotype refers to the physical/clinical expression/presentation/characteristics of the animal or disease state. My guys were all “wild type” meaning they’re just “normal” standard mice and we induced the “obese phenotype” (obese disease state with the associated characteristics and physical presentation associated with the disease) with the two high fat diets. 60% had a greater impact on inducing these changes compared to the control group than the 40% group.

no worries, I love talking about work! Nutrition is especially interesting given how relevant it is in our day to day lives and how complicated everything is between food itself, genetics and our gut microbiome. I could read about it all day, and not because I had to for two years!

60% produced a more dramatic phenotype and I remember it being the most popular diet in animal studies (I did all this 10 years ago so the details are a little fuzzy) so I’d probably go with that one.

Compliance wasn’t an issue since we we ran the study in mice and they all liked the food. they’re all basically clones so so it helps eliminate a LOT of variables. As expected we found the 60% diet induced a much more dramatic phenotype than the 40% but both induced obesity in general, but even ONLY having 60 vs 40% fat the differences were significant enough to make me reluctant to compare the two HFDs especially when you dive into microbiota stuff. I wouldn’t say its apples and oranges, more like apples and crab apples… or something.

We used purified high fat diets, one at 40% and one at 60% and compared the two. We had a whole other project where each group were supplemented with lentils but we I focussed on just the difference between those two diets where the only variable between them were the carb/fat percentage, they were otherwise the same/pure.

I did my masters thesis on high fat diets and while I was doing my lit review I realized there was no standard for what a “high fat diet” even is. There are SO many variables and its insane some of the logic leaps some studies come to to complete a narrative.

slam

Is that the same company that makes histology stuff? half my lab is Leica equipment

can confirm.

hell yeah

correlation and causation. even useless stats comparing apples and oranges, the numbers generated are only as good as the study design and methods.

what about switch 2

littering aaaaaaand?

slam

I’ve used it in car rides and its not that bad. its not amazing, but better than no sound.

same company that made a system that could always wirelessly pair with bluetooth headsets/ear buds but didn’t enable that function for 4 YEARS then randomly dropped a software update activating that feature. That will always blow my mind.

graphpad is my #1

videogames and pizza

We got another one for the wall of shame, grab the framing kit.